Invigentech INVI DNA 轉染試劑

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Invigentech INVI DNA 轉染試劑

INVI DNA Transfection Reagent
IV1214025 ， 0.25 ml
IV1214050 ， 0.5 ml
IV1214075 ， 0.75 ml
IV1214100， 1 ml
IV1214150 ， 1.5 ml
IV1214300 ， 3 ml

品牌：Invigentech

促銷：￥758.00~5958.00

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規格：

0.25ml 0.5ml 0.75ml 1ml 1.5ml 3ml

產品詳細

產品概述：

轉染效率高轉染效率高達96%，平均轉染效率高達70%。

細胞毒性小轉染后細胞存活率可高達98%，平均存活率高達80%。

實驗流程簡單轉染操作與傳統轉染試劑無任何不同，讓第一次使用該產品的用戶也能流暢操作。

產品名稱	貨號	規格	報價
INVI DNA轉染試劑	IV1214025	0.25 ml	￥ 758
INVI DNA轉染試劑	IV1214050	0.50 ml	￥ 1358
INVI DNA轉染試劑	IV1214075	0.75 ml	￥ 2458
INVI DNA轉染試劑	IV1214100	1.00 ml	￥ 2758
INVI DNA轉染試劑	IV1214150	1.50 ml	￥ 3158
INVI DNA轉染試劑	IV1214300	3.00 ml	￥ 5958

Invigentech（英克）簡介

美國 Invigentech（英克）公司是開發用于細胞培養的胎牛血清、細胞活力檢查、細胞篩選、細胞支原體清除、細胞轉染和組織再生的新型血清替代品全球＊＊＊之一；inviCELL?Platelet lysate提供無動物血清產品旨在支持廣泛的細胞擴增和生產，包括培養間充質干細胞和多種免疫細胞系等，為制藥公司或者生物技術公司提供無血清細胞培養規模生產服務；INVI DNA RNA Transfection Reagent為原代細胞、懸浮細胞、小動物活體轉染提供高品質轉染試劑；此外我們引進的新設施可生產高純度注射用水和生物處理解決方案，用于滿足任何細胞、科研和企業客戶需求，保證每個指定批次胎牛血清、細胞活力檢查、細胞篩選、細胞轉染等產品質量的穩定性，以加速安全有效的基于細胞和組織的治療研究、開發和商業化。

INVI DNA Transfection Reagent?

CatNo:IV1214

1.Description

INVI DNA Transfection Reagent? is a newly developed reagent for the transfection of DNA into eukaryotic cells. Advantages:

-The highest transfection efficiency in many cell types.

-DNA-INVI DNA Transfection Reagent?complexes can be directly added to cells in culture medium.

-It is not necessary to remove DNA-INVI DNA Transfection Reagent? complexes following transfection.

-The complexes can be removed after 4-6 hours by replacing with refresh medium (optional)

2.Contents and Storage

Contents: INVI DNA Transfection Reagent? is supplied in liquid form .

Storage : Store at 4oC. DO NOT FREEZE.

3.Transfection Procedure（24-Well Format）

A. For each transfection sample，prepare DNA-INVI DNA Transfection Reagent? complexes as follows:

a. Dilute DNA in 50 μl of Opti-MEM. Mix gently.

b. Mix INVI DNA Transfection Reagent? gently before use，then dilute the appropriate amount in 50 μl of Opti-MEM. Mix gently and incubate for 5 minutes at room temperature.

c. After the 5 minute incubation，combine the diluted DNA with the diluted INVI DNA Transfection Reagent?(total volume is 100 μl). Mix gently and incubate for 20 minutes at room temperature to allow the DNA-INVI DNA Transfection Reagent? complexes to form. The solution may appear cloudy，but this will not inhibit the transfection.

Note: DNA-INVI DNA Transfection Reagent? complexes are stable for at least 5 hours at room temperature.

B. Add the 100 μl of DNA-INVI DNA Transfection Reagent? complexes to each well.

C. Incubate the cells at 37oC in a CO2 incubator for 24-48 hours until they are ready to assay for transgene expression. It is not necessary to remove the complexes or change the medium; however，growth medium may be replaced after 4-6 hours without loss of transfection activity.

D. The table of Transfection Procedure:

Culture Vessel	Surface Area per Well (cm2)	Volume of Plating Medium	DNA (μg) and Dilution Volume (μl)	INVI DNA Transfection Reagent? (μl) and Dilution Volume (μl)
96-well	0.3	100 μl	0.2 μg in 25 μl	0.5 μl in 25 μl
24-well	2	500 μl	0.8 μg in 50 μl	2.0 μl in 50 μl
12-well	4	1 ml	1.6 μg in 100 μl	4.0 μl in 100 μl
35-mm	10	2 ml	4.0 μg in 250 μl	10 μl in 250 μl
6-well	10	2 ml	4.0 μg in 250 μl	10 μl in 250 μl
60-mm	20	5 ml	8.0 μg in 0.5 ml	20 μl in 0.5 ml
10-cm	60	15 ml	24 μg in 1.5 ml	60 μl in 1.5 ml

4.Important Guidelines

Follow these guidelines when performing transfections:

A.The ratio of DNA (in μg) : INVI DNA Transfection Reagent? (in μl) to use when preparing complexes should be 1:1 to 1:4for most cell lines.

B. It is CRITICAL to transfect cells at high cell density. 70-90% confluence the time of transfection is recommended to obtain high efficiency and expression levels and to minimize decreased cell growth associated with high transfection activity. Lower cell densities are suitable with optimization of conditions.

C. DO NOT add antibiotics to media during transfection as this will cause cell death.

5.Optimizing Transfection

To obtain the highest transfection efficiency and low non-specific effects，optimize transfection conditions by varying DNA and INVI DNA Transfection Reagent? concentrations， and cell number. Make sure that cells are greater than 90% confluent and vary DNA(μg)/INVI DNA Transfection Reagent? (μl) ratios from 1/0.5 to 1/5.